Some pseudo-scientists at JPAC/DPMO are still saying that it is not possible to get nuclear DNA from remains as old as from WWII. Here is a nuclear DNA testing paper published 7-years ago. As this peer-reviewed article shows, it was possible to get nuclear DNA from WWII remains back in 2007 and the science has advanced quite a bit since then. ALL 27 WWII CASES PRODUCED NUCLEAR DNA PROFILES. So, if someone claims it is not possible to get nuclear DNA from WWII remains that is a demonstratively false statement. Once again, this was 7-years ago and the science has moved forward quite a bit since then.
In addition, though not discussed in detail here, family references were located and IDs made. Thus, this type of system worked in the Balkan area of Europe for WWII missing.
DNA identification of skeletal remains from the World War II mass graves uncovered in Slovenia.
2007: Damir Marjanović; Adaleta Durmić-Pasić; Narcisa Bakal; Sanin Haverić; Belma Kalamujić; Lejla Kovacević; Jasmin Ramić; Naris Pojskić; Vedrana Skaro; Petar Projić; Kasim Bajrović; Rifat Hadziselimović; Katja Drobnic; Edwin Huffine; Jon Davoren; Dragan Primorac
Croatian medical journal 2007;48(4):513-9.
To present the joint effort of three institutions in the identification of human remains from the World War II found in two mass graves in the area of Škofja Loka, Slovenia.
The remains of 27 individuals were found in two small and closely located mass graves. The DNA was isolated from bone and teeth samples using either standard phenol/chloroform alcohol extraction or optimized Qiagen DNA extraction procedure. Some recovered samples required the employment of additional DNA purification methods, such as N-buthanol treatment. QuantifilerTM Human DNA Quantification Kit was used for DNA quantification. PowerPlex 16 kit was used to simultaneously amplify 15 short tandem repeat (STR) loci. Matching probabilities were estimated using the DNA View program.
Out of all processed samples, 15 remains were fully profiled at all 15 STR loci. The other 12 profiles were partial. The least successful profile included 13 loci. Also, 69 referent samples (buccal swabs) from potential living relatives were collected and profiled. Comparison of victims’ profile against referent samples database resulted in 4 strong matches. In addition, 5 other profiles were matched to certain referent samples with lower probability.
Our results show that more than 6 decades after the end of the World War II, DNA analysis may significantly contribute to the identification of the remains from that period. Additional analysis of Y-STRs and mitochondrial DNA (mtDNA) markers will be performed in the second phase of the identification project.
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